Dr. James Chan is a Staff Scientist in the Physics Division at Lawrence Livermore National Laboratory (LLNL) in Livermore, CA. In addition, he is a staff researcher at the NSF Center for Biophotonics Science and Technology (CBST) at the UC Davis Medical Center in Sacramento, CA. His research interest broadly encompasses the development of label-free spectroscopic and microscopic techniques for biomedical, biosensing, and chemical sensing applictions. Techniques of interest include vibrational spectroscopy (Raman, surface enhanced Raman, coherent anti-Stokes Raman,infrared) and second harmonic generation (SHG) microscopy.
Dr. Chan received his Ph.D. from the Chemical Engineering and Materials Science Dept at UC Davis in 2002, where he used a combination of confocal Raman and fluorescence spectroscopy to investigate the molecular level structural changes in glass induced by tightly focused ultrashort laser pulses. From 2003-2005, he held a postdoctoral appointment at Lawrence Livermore National Laboratory where he applied Raman and CARS spectroscopy for biological and biomedical research. Since 2005, as a Staff Scientist at LLNL, he has been involved with research developing and characterizing novel fiber-based chemical sensors based on vibrational spectroscopy (Raman, IR, SERS) for remote chemical sensing applications while continuing his biophotonics research at CBST.
Research projects in Dr. Chan's lab include:
- Single cell laser trapping Raman spectroscopy
- Raman and IR based optical fiber sensors
- SERS-based sensors
- Nonlinear microscopy (SHG, CARS)
Current CBST students mentored by Dr. Chan:
- Rui Liu, BIomedical Engineering
- Samir Awasthi, Biomecial Engineering
- Tobias Moritz, Biophysics Graduate Group
Selected List of Publications (from the past 3 years)
1. J.W. Chan, D.S. Taylor, T. Zwerdling, S.M. Lane, T. Huser, “Non-destructive identification of individual leukemia cells by laser trapping Raman spectroscopy,” Analytical Chemistry, 80, 2180-2187 (2008)
2. B.S. Kim, C.I. Lee, J.E. Christensen, J.W. Chan, T.R Huser, A.F.Tarantal, “Evaluation of age-related differences in growth, differentiation, and Raman spectroscopic profiles of rhesus monkey mesenchymal stem cells,” Stem Cells and Development, 17, 185-198 (2008)
3. W.F. Pearman, J.C. Carter, S.M. Angel, J.W. Chan, “Multi-pass capillary cell for enhanced Raman measurements of gases,” Applied Spectroscopy, 62, 285-289 (2008)
4. A. Lau, L. Lee, J.W. Chan, “An integrated optofluidic platform for Raman-activated cell sorting,” Lab on a Chip, 8, 1116-1120 (2008)
5. J.W. Chan, S. Fore, S. Wachsmann-Hogiu, T. Huser, “Raman spectroscopy and microscopy of individual cells and cellular components,” Lasers and Photonics Review, 2, 325-349 (2008)
6. W.F. Pearman, J.C. Carter, S.M. Angel, J.W. Chan, “Quantitative measurements of CO2 and CH4 using a multi-pass capillary cell,” Applied Optics, 47, 4627-4632 (2008)
7. J.W. Chan, D.S. Taylor, D.L Thompson, “The effect of cell fixation on the discrimination of normal and leukemia cells with laser tweezers Raman spectroscopy,” Biopolymers, 91, 132-139 (2008)
8. S. Kim, N. Menegazzo, C. Young, J. Chan, C. Carter, B. Mizaikoff, “Mid-infrared trace gas analysis with single-pass FT-IR hollow waveguide gas sensors,” Applied Spectroscopy, 63, 331-337 (2009)
9. J.W. Chan, D.K. Lieu, T. Huser, R.A. Li, “Label-free, non-invasive spectroscopic separation of human embryonic stem cells (hESCs) and their cardiac derivatives,” Analytical Chemistry, 81, 1324-1331 (2009)
10. J.W. Chan, D.K. Lieu, “Label-free biochemical characterization of stem cells using vibrational spectroscopy,” J. Biophotonics, 2, 656-668 (2009)
11. J.W. Evans, R.J. Zawadzki, R. Liu, J.W. Chan, S.M. Lane, J.S. Werner “Optical coherence tomography and Raman spectroscopy of the ex-vivo retina,” J Biophotonics, 2, 398-406 (2009)
12. T. J. Moritz, J.A. Brunberg, D.M. Krol, S. Wachsmann-Hogiu, S.M. Lane, J.W. Chan, “Characterisation of FXTAS related isolated intranuclear protein inclusions using laser tweezers Raman spectroscopy,” J. Raman Spectroscopy, 41, 33-39 (2010)
13. T.J. Moritz, D.S. Taylor, C.R. Polage, D.M. Krol, S.M. Lane, J.W. Chan, “The effect of Cefazolin treatment on the non-resonant Raman signatures of the metabolic states of Escherichia Coli cells,” Analytical Chemistry, 82, 2703-2710 (2010)
Contact:
James Chan
Physics Division
Lawrence Livermore National Laboratory
7000 East Ave, L-211
Livermore, CA 94550
Phone: 925-423-3565
Email: chan19@llnl.gov
